Comprehensive Expression and Prognosis Analyses of ITGB1 in AML

Author Info

Xinyi Zhou Nan Jin Bao-An Chen

Corresponding Author

Bao-An Chen
Department of Hematology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu Province, China

A B S T R A C T

Background: Acute myeloid leukemia (AML) is a dangerous type of leukemia. The emergence of multidrug resistance (MDR) and recurrence limits the prognosis and survival of patients. In recent studies, we have known that the bone marrow microenvironment was closely related to the poor prognosis of AML. However, the underlying mechanisms are still far from fully understood. By utilizing the bioinformatics analysis, we screened out integrin B1 (ITGB1) as the hub gene, which is associated with the bone marrow microenvironment mediated changes of AML cells, with expression profile GSE73157 downloaded from the National Center for Biotechnology Information-Gene Expression Omnibus (NCBI-GEO) database. Methods: R studio software was used to screen out candidate hub genes and further visualize the differential expression. R package ‘limma’ was to find out differentially expressed genes (DEGs). Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were conducted by R package “clusterProfiler”. Furthermore, the protein-protein interaction (PPI) network was also performed by the online tool STRING and software Cytoscape. Last but not least, online tools PrognoScan and GEPIA were utilized for the evaluation of the clinical significance of the selected hub gene. P and Cox p-value <0.05 was considered to be statistically significant. Results: ITGB1 was filtrated as the only hub gene in this profile. We found that patients with high expression of ITGB1 had significantly longer overall survival (OS) than those with low expression (COX p-value= 0.016730). Besides, the expression of the ITGB1 gene in AML patients is lower than that in normal people significantly (p-value<0.01). Conclusion: We identified ITGB1 as a key gene in the bone marrow microenvironment mediated poor prognosis in AML. The down-regulated expression of ITGB1 was related to AML patients’ poor outcomes. ITGB1 may be a potential marker for predicting and guiding AML treatment.

Article Info

Article Type

Research Article

Publication history

Received: Wed 14, Jul 2021
Accepted: Thu 29, Jul 2021
Published: Thu 12, Aug 2021

Copyright

© 2023 Bao-An Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.COR.2021.08.04