Electrophysiological and Morphological Studies of SOD1 Transgenic Mice: An Animal Model of ALS

Author Info

N Hori Y Tan Z Xu N Akaike David O. Carpenter

Corresponding Author

David O. Carpenter
Institute for Health and the Environment; University at Albany, SUNY, Rensselaer, NY, USA

A B S T R A C T

Amyotrophic lateral sclerosis (ALS) is a disease where upper and lower motor neurons die, and it is often associated with mutations of superoxide dismutase 1 (SOD1). We have used mouse models to compare physiologic and morphologic characteristics of cervical motor neurons in wild-type and mutant animals. Slices of the cervical spinal cord were prepared from old wild-type and mutant G93A and G85R mice, and intracellular recordings of membrane potential, resistance and responses to application of excitatory neurotransmitters were studied. Some motor neurons were injected with Lucifer Yellow for morphological analysis. There were no significant differences between membrane potential in the SOD1 mutants and aged wild-type mice, but membrane resistance was somewhat higher in the mutant motor neurons. Dendrites of the mutant motor neurons were not responsive to ionophoretic application of excitatory amino acids, although the cell body responded strongly. In Lucifer-filled cells, the dendrites were found to disappear. Mutant motor neurons were sometimes spontaneously active. Responses of mutant motor neurons to perfused glutamate with varying calcium concentrations in the Ringer’s solution were different from those of the wild-type cells.

Article Info

Article Type

Research Article

Publication history

Received: Mon 16, Aug 2021
Accepted: Tue 31, Aug 2021
Published: Sat 02, Oct 2021

Copyright

© 2023 David O. Carpenter. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.JBN.2021.01.03