Table 1: The expression of selected genes in AZ and GLY treated for 6- and 12-hour treatment calculation using-ΔΔCT.
|
Genes |
Groups |
Average CT ± SD |
Fold changes (2^- ΔΔCT) |
Fold differences (-ΔΔCT) |
|
MITF |
6 hour AZ treated |
18.3 ± 0.318 |
0.831 |
-0.265 |
|
12 hour AZ treated |
17.5 ± 0.216 |
0.527 |
-0.921 |
|
|
6 hour GLY treated |
18.8 ± 0.394 |
0.577 |
-0.792 |
|
|
12 hour GLY treated |
17.3 ± 0.241 |
0.607 |
-0.719 |
|
|
Untreated (6 hour) |
18.6 ± 0.273 |
1 |
0 |
|
|
Untreated (12 hour) |
17.5 ± 0.0847 |
1 |
0 |
|
|
MC1R |
6 hour AZ treated |
22.0 ± 0.379 |
0.880 |
-0.182 |
|
12 hour AZ treated |
22.6 ± 0.374 |
0.430 1 |
-1.21 |
|
|
6 hour GLY treated |
22.5 ± 0.216 |
0.615 |
-0.700 |
|
|
12 hour GLY treated |
22.5 ± 0.200 |
0.434 1 |
-1.20 |
|
|
Untreated (6 hour) |
22.4 ± 0.363 |
1 |
0 |
|
|
Untreated (12 hour) |
22.3 ± 0.594 |
1 |
0 |
|
|
TYR |
6 hour AZ treated |
21.8 ± 0.183 |
0.533 |
-0.906 |
|
12 hour AZ treated |
22.2 ± 0.234 |
0.337 1 |
-1.56 |
|
|
6 hour GLY treated |
22.3 ± 0.184 |
0.391 1 |
-1.35 |
|
|
12 hour GLY treated |
22.0 ± 0.215 |
0.389 1 |
-0.389 |
|
|
Untreated (6 hour) |
21.5 ± 0.218 |
1 |
0 |
|
|
Untreated (12 hour) |
21.6 ± 0.214 |
1 |
0 |
|
|
TYRP1 |
6 hour AZ treated |
19.5 ± 0.313 |
0.612 |
-0.706 |
|
12 hour AZ treated |
20.2 ± 0.429 |
0.335 1 |
-1.57 |
|
|
6 hour GLY treated |
19.6 ± 0.235 |
0.590 |
-0.760 |
|
|
12 hour GLY treated |
19.7 ± 0320 |
0.452 1 |
-1.14 |
|
|
Untreated (6 hour) |
19.4 ± 0.104 |
1 |
0 |
|
|
Untreated (12 hour) |
19.5 ± 0.260 |
1 |
0 |
|
|
TYRP2 |
6 hour AZ treated |
22.7 ± 0.139 |
0.311 1 |
-1.68 |
|
12 hour AZ treated |
21.9 ± 0.109 |
0.385 1 |
-1.37 |
|
|
6 hour GLY treated |
22.7 ± 0.222 |
0.311 1 |
-1.68 |
|
|
12 hour GLY treated |
21.3 ±0.248 |
0.569 |
-0.811 |
|
|
Untreated (6 hour) |
21.6 ± 0.340 |
1 |
0 |
|
|
Untreated (12 hour) |
21.5 ± 0.0865 |
1 |
0 |