Table 2: Discrimination thresholds of sniffer mice for pairs of human urine mixtures in a Y-maze.

Urine mixture pair	Dilution types	Discrimination thresholds (D.T.) of urine mixtures	Cancer	Stage	Gleason score / Grade	Age	Gender	Sampling period (5 days)
pre-RP P:U_m	equal-occult blood	2.0 × 10^-12 (9.2 × 10^-15)	prostate	5 Ⅱ	7.2	62–77	♂	(−41)–(−1)
post-RP P:U_m			none	–				1–33
pre-RP Pe:U_m	equal-occult blood	1.0 × 10^-9* (3.6 × 10^-12)*	prostate (ET)	4Ⅱ+ 1Ⅲ	6.0	58–80	♂	(−41)–(−1)
post-RP Pe:U_m			none	–				1–29
pre-TUR N:U_m	equal-occult blood	7.7 × 10^-10 (5.7 × 10^-10)	bladder	5 I	3H + 2L	69–91	♂	(−53)–(−1)
post-TUR N:U_m			none	–				8–127
healthy H1–3 U_m	equally diluted	1.0 × 10^-5 (2.6 × 10^-6)	none	–	–	52–75	♂	(−17)–(−1)^¶
healthy H4–6 U_m			none	–				0–11^#

RP: Radical Prostatectomy; ET: neoadjuvant Endocrine Therapy; TUR: Transurethral Resection of bladder tumor. P:U_m: P-series Urine mixture.

(U_m) is an equal-volume mixture of 25 urine samples from five patients with prostate cancer: P2, P5, P6, P8, and P9 on five different days. Pe:U_m, Pe-series U_m is an equal-volume mixture of 25 urine samples from five patients: Pe1, Pe3, Pe7, Pe11, and Pe14 on five different days. The discrimination thresholds of sniffer mice were obtained as the lowest urine concentrations with the average values of the %Correct for 4-8 mice (18 trials per mouse) greater than 61.5% or 58.3% that were significantly different from chance level (P = 0.05 for 72 or 144 trials). Estimated threshold concentration in parenthesis was calculated by the odds ratio (%Correct to chance) of the logit, with P = 76.65% and linear regression models of %Correct. Urine sample was diluted in distilled water (v/v). *Preliminary result for four mice (the lowest concentration with the %Correct greater than 61.55%, P = 0.05 for 72 trials). **Thresholds for bladder cancer N:U_m and healthy H1–3 vs. H4–6 U_ms were previously reported [11].