The Effects of Vitamin D3, Vitamin B12, and Vitamin K on the Differentiation of Porcine Adipose-Derived Stem Cells (ASC) into Osteoblasts

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Author Info

Bane T.A. Bertels J.C. Matthew B. Wheeler Polkoff K.M. Rubessa M.

Corresponding Author

Matthew B. Wheeler
Department of Animal Sciences, University of Illinois, Urbana-Champaign, Urbana, Illinois, USA

A B S T R A C T

Large bone defects present a tremendous challenge to the treating surgeon. Tissue engineering using scaffolds of various sizes and shapes that contain stem cells and other osteoinductive molecules offer a potential solution to this difficult problem. The aim of this project was to evaluate if standard osteogenic medium infused with either vitamin D3, vitamin K, or vitamin B12 influences the differentiation of adiposederived stem cells (ASC) into osteoblasts. Only the 100 nM vitamin D3 treatment had a positive influence on ASC differentiation into osteoblasts. Moreover, we found higher levels (statistically significant) of osteocalcin production in all vitamin groups compared with the standard osteogenic medium.

Article Info

Article Type

Research Article

Publication history

Received: Mon 20, Apr 2020
Accepted: Tue 05, May 2020
Published: Mon 11, May 2020

Copyright

© 2023 Matthew B. Wheeler. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.JBEM.2020.01.01