Detection of 2-Dodecylcyclobutanone in Low-Dose Irradiated (0.5 Gy) Goose Fat by Triple-Quad GC-MS/MS

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Author Info

Gesine Mandt Jenifer Wohlers Peter Stolz Roya Bornhütter Uwe Geier

Corresponding Author

Peter Stolz
Forschungsinstitut KWALIS gGmbH, Dipperz, Germany

A B S T R A C T

Aim of this work was the development of a sensitive procedure to detect low-dose irradiation in the scale of 0.5 Gy to 20 Gy in goose fat by detection of 2-dodecylcyclobutanone (2-DCB). The general procedure to detect 2-alkylcyclobutanone (2-ACB) according to EN 1785 was optimized by using acetonitrile for extraction of 2-DCB from the lipid fraction before clean-up with silica gel. Gas chromatographic separation was realized by a 15 m TG-5 HT column, and detection was done by using a triple-quad tandem-mass spectrometer in SRM-mode. At the irradiation level of 2 Gy, the optimized procedure showed high repeatability (3.9%), a good intermediate precision (20.9%) and an acceptable recovery rate of 57.8%, with 0.25 ng 2-DCB per gram of fat. With this procedure, a reliable proof of 2-DCB was possible for the irradiation intensities 20, 5 and 2 Gy for three mass transitions; for 0.5 Gy, a proof was possible for one mass transition.

Article Info

Article Type

Research Article

Publication history

Received: Mon 11, May 2020
Accepted: Fri 29, May 2020
Published: Wed 10, Jun 2020

Copyright

© 2023 Peter Stolz. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.JFNM.2020.02.01