Proteomics Approach (iTRAQ) to Test Subchronic and Chronic Toxicity Effects of Mice Exposed to Drinking Water Source from Yangtze River Delta

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Author Info

Jie Sun Liqun Xing Houcheng Cheng

Corresponding Author

Jie Sun
Suzhou Capital Greinworth Environmental Protection Technology Co., Ltd., Suzhou, China

A B S T R A C T

Objective: To investigate the combined effects of the pollutants in source of drinking water for local populations, mice models were used to test the subchronic toxicity for three months and chronic toxicity for six months. Methods and Results: By using proteomics approach (iTRAQ), we had identified 29 differentially expressed proteins, which were related to amino acids metabolism, cell skeleton reorganization, apoptosis and cancer genesis. Moreover, 12 out of these 29 identified proteins are located in mitochondria, indicating drinking water source from Yangtze River Delta may exert oxidative damage stress to liver cells. All these cellular processes had been verified by hepatic histopathology, real time-PCR, flow cytometry test and our previous metabolic profile results. Conclusion: All these tests indicated that drinking water source from Yangtze River Delta could accelerate aging, enhance apoptosis and carcinogenesis risk.

Article Info

Article Type

Research Article

Publication history

Received: Sat 19, Dec 2020
Accepted: Sat 09, Jan 2021
Published: Mon 18, Jan 2021

Copyright

© 2023 Jie Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.JSO.2021.01.01