Calcium Phosphate Treatment Enhances Osteogenic Differentiation of Porcine Adipose-Derived Stem Cells on Fibrin Scaffolds

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Author Info

Aaron J. Maki R. A. Chanaka Rabel Matthew B. Wheeler

Corresponding Author

Matthew B. Wheeler
Department of Animal Sciences, University of Illinois, Urbana-Champaign, Urbana, Illinois, USA

A B S T R A C T

This study was designed to develop a fibrin scaffold for optimum in vitro osteogenic differentiation of porcine ASCs. Fibrin scaffolds physically and chemically modified to be stiffer or have higher concentrations of calcium and phosphate ions were hypothesized to enhance osteogenic differentiation compared to control fibrin scaffolds. Treatments during coagulation resulted in six scaffold types for comparison: whole blood controls, red blood cell lysis buffer, calcium chloride, calcium hydrogen phosphate, vacuum, and mechanical compression. Based on the results, fibrin supplemented with granules of calcium hydrogen phosphate (CaHPO4) was determined to be the most suitable formulation of those examined. Using calcium phosphate resulted in a fibrin scaffold that coagulated faster (p = 0.022), had a rougher surface, higher stiffness, and desirable properties for practical use during surgical operations and scaffolds used in bone tissue engineering. Further, osteogenic differentiation was enhanced on scaffolds treated with calcium phosphate. In addition, fibrin scaffolds treated with RBC lysis buffer were also stiffer than untreated controls. These results are partly explained by ASC attachment and fibrin polymerization during coagulation.

Article Info

Article Type

Research Article

Publication history

Received: Thu 04, May 2023
Accepted: Thu 18, May 2023
Published: Tue 13, Jun 2023

Copyright

© 2023 Matthew B. Wheeler. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.RGM.2023.02.01