TY - JOUR
AR - DOBCR-2020-6-105
TI - Enamel Matrix Derivative and TGF-Beta 1 Target Genes in Human Tongue Carcinoma Cells
AU - Matti, Mauramo
AU - Suvi-Tuuli , Vilen
AU - Timo , Sorsa
AU - Tuula , Salo
JO - Dental Oral Biology and Craniofacial Research
PY - 2020
DA - Thu 31, Dec 2020
SN - 2613-4950
DO - http://dx.doi.org/10.31487/j.DOBCR.2020.06.05
UR - https://www.sciencerepository.org/enamel-matrix-derivative-and-tgf-beta-1-target-genes-in-human_DOBCR-2020-6-105 
KW - Enamel matrix derivative, TGF-beta, oral carcinoma, microarray, gene expression, MMP
AB - Enamel matrix derivative (EMD) can enhance proliferation and migration of different oral cell lines,
including malignant oral carcinoma cells, in vitro and in vivo. The composition of EMD is not known, but
part of the effects have been postulated to be caused by transforming growth factor-beta-1 (TGF-beta 1).
This study aimed to compare target genes of EMD and TGF-beta 1 on highly malignant oral carcinoma
HSC-3 cells. Microarrays were used to examine differentially expressed genes in HSC-3 cells after 6h and
24h incubations with EMD (200 µg/ml) or TGF-beta 1 (10 ng/ml). Gene Ontology (GO) enrichment analysis
of the regulated genes was also conducted. After 6h and 24h of EMD treatments 42 and 12 genes,
respectively, were statistically significantly (P<0.05) up- or down-regulated. However, as many as 393 and
346 genes were statistically significantly (P<0.05) up- or down-regulated by TGF-beta 1. Among the most
up-regulated genes by both of the study reagents were MMP-9 and -10. The expression of MMP-10 by
EMD treated carcinoma cells was also verified in protein level. In conclusion, TGF-beta 1 regulates more
and mostly different genes compared with EMD, but both regulate the expression of matrix
metalloproteinase genes in oral carcinoma cells.