TY - JOUR
AR - IJCST-2021-2-101
TI - BDE-209 and TCDD Modulate the Expression and Activity of ATP-Binding  Cassette (ABC) Transporters in Murine Melanoma Cells (B16-F1)
AU - Micheli, de Marchi
AU - Erick E., Moggio
AU - Francisco, Filipak Neto
AU - Patricia E.M., Brito
AU - Benisio, F. Silva Filho
AU - Ciro A. Oliveira, Ribeiro
JO - International Journal of Cancer Science and Therapy
PY - 2021
DA - Thu 02, Sep 2021
SN - 
DO - http://dx.doi.org/10.31487/j.IJCST.2021.02.01
UR - https://www.sciencerepository.org/bde-209-and-tcdd-modulate-the-expression-and-activity_IJCST-2021-2-101 
KW - Dioxin, BDE-209, ATP-binding cassette, melanoma, B16-F1
AB - Persistent organic pollutants (POPs) may alter tumor cells phenotype, possibly increasing malignancy, but 
there is a lack of studies investigating the mechanisms by which POPs may affect tumor cells. The ATP-Binding Cassette (ABC) transporter proteins are a widely studied component of drug resistance and tumor 
progression. We hypothesized that the levels of BDE-209 and TCDD detected in human serum can modulate 
the gene expression or activity of ATP-binding cassette (ABC) transporters in murine melanoma (B16-F1) 
cells. In this study, we observed an upregulation of the ABCB1 and ABCC4 (24 h) genes followed by an 
increased protein activity after BDE-209 15 day-exposure. We also observed that cells exposed to TCDD 
showed an upregulation of ABCB5, ABCC1 and ABCC4 genes (24 h) and change of protein activity after 15 
days of exposure. These findings suggest that BDE-209 and TCDD can regulate the phenotype of B16-F1 
cells by interfering with the expression and activity of ATP-binding cassette (ABC) transporters. This
investigation revealed that environmental pollutants might intervene and modify cells’ resistance to 
chemotherapy and cancer prognosis.