TY - JOUR
AR - JBEM-2020-1-101
TI - The Effects of Vitamin D3, Vitamin B12, and Vitamin K on the Differentiation of Porcine Adipose-Derived Stem Cells (ASC) into Osteoblasts
AU - Bane , T.A.
AU - Bertels , J.C.
AU - Matthew B., Wheeler
AU - Polkoff , K.M.
AU - Rubessa , M.
JO - Journal of Biology Engineering and Medicine
PY - 2020
DA - Mon 11, May 2020
SN - 
DO - http://dx.doi.org/10.31487/j.JBEM.2020.01.01
UR - https://www.sciencerepository.org/the-effects-of-vitamin-d3-vitamin-b12-and-vitamin-k_JBEM-2020-1-101 
KW - ASC, differentiation, osteogenesis, osteocalcin, vitamins, regeneration
AB - Large bone defects present a tremendous challenge to the treating surgeon. Tissue engineering using
scaffolds of various sizes and shapes that contain stem cells and other osteoinductive molecules offer a
potential solution to this difficult problem. The aim of this project was to evaluate if standard osteogenic
medium infused with either vitamin D3, vitamin K, or vitamin B12 influences the differentiation of adiposederived stem cells (ASC) into osteoblasts. Only the 100 nM vitamin D3 treatment had a positive influence
on ASC differentiation into osteoblasts. Moreover, we found higher levels (statistically significant) of
osteocalcin production in all vitamin groups compared with the standard osteogenic medium.