TY - JOUR
AR - JFNM-2020-2-103
TI - Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Method for the Detection of Salmonella spp. in Terms of Sensitivity and Applicability
AU - Deguo , Wang
AU - Meng , Zhang
AU - Yanhong, Liu
AU - Yongzhen , Wang
JO - Journal of Food Nutrition and Metabolism
PY - 2020
DA - Fri 03, Jul 2020
SN - 2674-2411
DO - http://dx.doi.org/10.31487/j.JFNM.2020.02.03
UR - https://www.sciencerepository.org/evaluation-of-a-loop-mediated-isothermal-amplification-lamp-method-for-the_JFNM-2020-2-103 
KW -  Salmonella spp, loop-mediated isothermal amplification (LAMP), detection, sensitivity
AB - Salmonella spp. are important food-borne pathogens that can cause diseases in humans. Many detection
methods have been established in Salmonella spp. using loop-mediated isothermal amplification (LAMP)
or reverse transcription loop-mediated isothermal amplification (RT-LAMP). The detection limits of these
assays varied from 1 CFU/reaction to 104 CFU/reaction, from 100 fg genomic DNA/reaction to 10 pg
genomic DNA/reaction, or from 2.0×101 CFU/mL to 107 CFU/mL for food samples. In this study, LAMP
assays were developed using genomic DNA for the detection of Salmonella spp. Two sets of LAMP primers
were designed using the invA gene and the 16S-23S rRNA intergenic spacer region (ITS) of S. enterica as
the target sequences for two LAMP assays. The detection limits of the two methods were respectively 20
pg S. enterica DNA/reaction and 10 pg S. enterica DNA/reaction at the optimized temperature, and the
LAMP methods were of high repeatability and specificity for S. enterica detection. This study provides a
baseline for the application of LAMP for the detection of food-borne pathogenic bacteria.