TY - JOUR
AR - JSO-2021-1-101
TI - Proteomics Approach (iTRAQ) to Test Subchronic and Chronic Toxicity Effects of Mice Exposed to Drinking Water Source from Yangtze River Delta
AU - Jie , Sun
AU - Liqun, Xing
AU - Houcheng , Cheng
JO - Journal of Surgical Oncology
PY - 2021
DA - Mon 18, Jan 2021
SN - 2674-3000
DO - http://dx.doi.org/10.31487/j.JSO.2021.01.01
UR - https://www.sciencerepository.org/proteomics-approach-itraq-to-test-subchronic-and-chronic-toxicity_JSO-2021-1-101 
KW - Proteomics, iTRAQ, subchronic and chronic toxicity, oxidative damage, drinking water source
AB - Objective: To investigate the combined effects of the pollutants in source of drinking water for local
populations, mice models were used to test the subchronic toxicity for three months and chronic toxicity for
six months.
Methods and Results: By using proteomics approach (iTRAQ), we had identified 29 differentially
expressed proteins, which were related to amino acids metabolism, cell skeleton reorganization, apoptosis
and cancer genesis. Moreover, 12 out of these 29 identified proteins are located in mitochondria, indicating
drinking water source from Yangtze River Delta may exert oxidative damage stress to liver cells. All these
cellular processes had been verified by hepatic histopathology, real time-PCR, flow cytometry test and our
previous metabolic profile results.
Conclusion: All these tests indicated that drinking water source from Yangtze River Delta could accelerate
aging, enhance apoptosis and carcinogenesis risk.