TY - JOUR
AR - RGM-2018-2-107
TI - Overexpression of BMP-2 in mesenchymal stem cells with amplifying virus-like particles
AU - Biao, He
AU - Christina, Elling
AU - Huiling, Wei
AU - Seth , Andrews
AU - Steve, Stice
JO - International Journal of Regenerative Medicine
PY - 2018
DA - Tue 28, Aug 2018
SN - 2613-5914
DO - http://dx.doi.org/10.31487/j.RGM.2018.02.007
UR - https://www.sciencerepository.org/overexpression-of-BMP-2-in-mesenchymal-stem-cells-with-amplifying-virus-like-particles_RGM-2-107 
KW - mesenchymal stem cells, parainfluenza virus 5, genetic therapy, bone morphogenetic protein-2
AB - There are numerous strategies under development to supplement or replace bone grafts. Among these synthetic options, growth factors such as bone morphogenetic protein (BMP-2) are often used. However, current burst delivery strategies have been associated with a number of complications. Transplanting cells engineered to over-express osteoinductive proteins over a longer period of time is a possible solution to this problem. In this study, we demonstrated the use of a parainfluenza virus 5 (PIV-5) vector (amplifying virus-like particles, AVLP) to induce expression of exogenous proteins in mesenchymal stem cells (MSC). We obtained extended expression of enhanced green fluorescent protein eGFP in transduced MSCs, as well as selection and maintenance of eGFP+ cells. We also attained expression of BMP-2 on a shorter timescale using this method. We conclude that AVLP is a promising tool for genetic engineering, both in regenerative medicine and other applications.