TY - JOUR
AR - RGM-2018-1-104
TI - Ethyl acetate fraction from Passiflora foetida promotes rat femoral fracture healing by the BMP-2 signaling pathway
AU -  Priyanka , Kothari
AU - Anirudha , Karvande
AU - Ashish Kumar , Tripathi
AU - Naseer , Ahmad
AU - Priyanka, Kushwaha
AU - Raju , Chillara
AU - Rakesh , Maurya
AU - Ritu , Trivedi
AU - Sulekha , Adhikary
JO - International Journal of Regenerative Medicine
PY - 2018
DA - Tue 15, May 2018
SN - 2613-5914
DO - http://dx.doi.org/10.31487/j.RGM.2018.10.004
UR - https://www.sciencerepository.org/ethyl-acetate-fraction-from-passiflora-foetida-promotes-rat-femoral-fracture-healing-by-the-BMP-2-signaling-pathway_RGM-1-104 
KW - Bone regeneration, trabecular microarchitecture, micro-computed tomography
AB - Aim: To evaluate the effect of ethyl acetate fraction (EAF) from Passiflora foetida on bone regeneration
following bone and marrow injury.
Materials and methods: EAF was administered for two weeks at 50, 100 and 200mg/kg doses orally to
adult female Sprague-Dawley rats having a drill-hole injury in femur. Analysis of calcein labelling, BMD,
micro-architectural parameters, tissue morphology at the drill-hole site, and serum level of bone turnover
markers, mineralized nodule formation, expression of osteogenic genes and localization of BMP-2 protein
was performed.
Results: EAF dose-dependently accelerated bone regeneration mainly by increasing BMD at the fracture
site, serum levels of PINP, OCN, and also mineralized nodule formation in BMCs. In addition, EAF also
enhanced microarchitecture of the regenerating bone evident from increased bone volume fraction,
trabecular thickness, trabecular number, connective density and decreased trabecular separation and degree
of anisotropy. The mechanism studies, EAF accelerated fracture healing in rats by the recruitment of
osteoblasts through up-regulation of the BMP-2 signaling pathway at the fracture site.
Conclusion: EAF accelerated fracture healing in rats by the recruitment of osteoblasts through upregulation of the BMP-2 signaling pathway at the fracture site, therefore could be taken as an alternative
therapy for fracture healing.