Anders  Høgset,Henry  Hirschberg,Jimmy Nguyen Le,Kristian  Berg,Odrun A  Gederaas,Steen J  Madsen,
Ultrasonic Activation of the Sonosensitizer Fimaporfin Enhances the Efficacy of Chemotherapy: An In Vitro Study on Rat Glioma Cells
Clinical Oncology and Research
2020
2613-4942
http://dx.doi.org/10.31487/j.COR.2020.05.10
https://www.sciencerepository.org/ultrasonic-activation-of-the-sonosensitizer-fimaporfin-enhances-the-efficacy-of-chemotherapy_COR-2020-5-110 
Abstract: Activation of sonosensitizers via focused ultrasound, i.e., sonodynamic therapy, has been proposed as an
alternative to light-activated photodynamic therapy for the treatment of a number of conditions from cancer
to bacterial infections. The use of focused ultrasound allows treatment to sites buried deep within tissues,
overcoming one of the main limitations of light-based modalities. Photochemical internalization is a
technique that utilizes the photochemical properties of photodynamic therapy for the release of trapped
endo-lysosomal macromolecules into the cell cytoplasm, greatly enhancing their efficacy. We have
examined ultrasonic activation of disulfonated tetraphenyl chlorin (fimaporfin) together with the anti-cancer
agent bleomycin, termed sonochemical internalization, as an alternative to light-activated photochemical
internalization. Our results indicate that, compared to drug or focused ultrasound treatment alone, focused
ultrasound activation of fimaporfin together with BLM significantly inhibits the viability of glioma
monolayers and the treated cells’ ability to form clonogenic colonies.
Keywords: Fimaporfin, sonochemical internalization, sonodynamic therapy, glioma cell line, bleomycin