Gesine  Mandt,Jenifer  Wohlers,Peter Stolz,Roya  Bornhütter,Uwe  Geier,
Detection of 2-Dodecylcyclobutanone in Low-Dose Irradiated (0.5 Gy) Goose Fat by Triple-Quad GC-MS/MS
Journal of Food Nutrition and Metabolism
2020
2674-2411
http://dx.doi.org/10.31487/j.JFNM.2020.02.01
https://www.sciencerepository.org/detection-of-2-dodecylcyclobutanone-in-low-dose-irradiated-05-gy-goose-fat_JFNM-2020-2-101 
Abstract: Aim of this work was the development of a sensitive procedure to detect low-dose irradiation in the scale
of 0.5 Gy to 20 Gy in goose fat by detection of 2-dodecylcyclobutanone (2-DCB). The general procedure
to detect 2-alkylcyclobutanone (2-ACB) according to EN 1785 was optimized by using acetonitrile for
extraction of 2-DCB from the lipid fraction before clean-up with silica gel. Gas chromatographic separation
was realized by a 15 m TG-5 HT column, and detection was done by using a triple-quad tandem-mass
spectrometer in SRM-mode. At the irradiation level of 2 Gy, the optimized procedure showed high
repeatability (3.9%), a good intermediate precision (20.9%) and an acceptable recovery rate of 57.8%, with
0.25 ng 2-DCB per gram of fat. With this procedure, a reliable proof of 2-DCB was possible for the
irradiation intensities 20, 5 and 2 Gy for three mass transitions; for 0.5 Gy, a proof was possible for one
mass transition.Keywords:  Limit of detection, 2-ACB, 2-DCB, x-ray irradiation, EN 1785, GC-MS/MS