Biao He,Christina Elling,Huiling Wei,Seth  Andrews,Steve Stice,
Overexpression of BMP-2 in mesenchymal stem cells with amplifying virus-like particles
International Journal of Regenerative Medicine
2018
2613-5914
http://dx.doi.org/10.31487/j.RGM.2018.02.007
https://www.sciencerepository.org/overexpression-of-BMP-2-in-mesenchymal-stem-cells-with-amplifying-virus-like-particles_RGM-2-107 
Abstract: There are numerous strategies under development to supplement or replace bone grafts. Among these synthetic options, growth factors such as bone morphogenetic protein (BMP-2) are often used. However, current burst delivery strategies have been associated with a number of complications. Transplanting cells engineered to over-express osteoinductive proteins over a longer period of time is a possible solution to this problem. In this study, we demonstrated the use of a parainfluenza virus 5 (PIV-5) vector (amplifying virus-like particles, AVLP) to induce expression of exogenous proteins in mesenchymal stem cells (MSC). We obtained extended expression of enhanced green fluorescent protein eGFP in transduced MSCs, as well as selection and maintenance of eGFP+ cells. We also attained expression of BMP-2 on a shorter timescale using this method. We conclude that AVLP is a promising tool for genetic engineering, both in regenerative medicine and other applications.Keywords: mesenchymal stem cells, parainfluenza virus 5, genetic therapy, bone morphogenetic protein-2