Alessandro Gennai,Piero  Tesauro,Mattia  Colli,Silvia  Zia, Barbara  Roda,Andrea  Zattoni,
Infranatant Portion of Microfragmented Adipose Tissue: A Promising Source of SVF for the Management of Androgenetic Alopecia
International Journal of Regenerative Medicine
2021
2613-5914
http://dx.doi.org/10.31487/j.RGM.2021.01.01
https://www.sciencerepository.org/infranatant-portion-of-microfragmented-adipose-tissue-a-promising-source_RGM-2021-1-101 
Abstract: Aim: The purpose of this article is to prove the possibility to transfer a good amount of cells of the SVF
(and ADSCs) in the infranatant portion of microfragmented adipose tissue.
Method: The adipose tissue harvesting procedure is performed under local anaesthesia. The adipose tissue
was harvested with a 2 mm diameter microperforated cannula with 1 mm side port holes, mounted inside
the special patented guide. Both cannula and guide are included in the SEFFIHAIR™ medical device. Once
the adipose tissue is harvested, it is gently washed, and it was divided in two specimens: (EMU) the tissue
was emulsified with 20 passages from one syringe to another and (CTRL) the tissue didn’t undergo any
emulsification.
Results: The emulsification procedure liberated alive and proliferating cells and we observed that the
specimens derived with a 1 mm side port hole cannula and then emulsified (EMU) showed a higher number
of cells in the infranatant part compared to the one derived from the control tissue without any (1 EMU vs.
1EMU infra).
Conclusion: The use of microcannulas, in combination with a mechanical digestion by an emulsification
procedure and centrifuge, could ease SVF cells isolation for regenerative treatment and could also be
performed in a medical facility.Keywords:  Androgenetic alopecia, hair loss regenerative therapy, hair transplantation, adipose-derived stem cells, autologous fat transfer, stromal-vascular fraction, regeneration clinical applications