The Effects of Vitamin D3, Vitamin B12, and Vitamin K on the Differentiation of Porcine Adipose-Derived Stem Cells (ASC) into Osteoblasts

Review Data

Purpose and Significance of Study: The authors studied the effects of vitamins D3, B12 and K on the differentiation of adipose-derived stem cells into osteoblasts in standard osteogenic medium in pigs. The results are of interest to tissue engineering with respect to large bone defects.

 

Fit with Scope of Journal: The manuscript is of high interest for the Journal of Biology Engineering and Medicine.

The manuscript contains a few minor errors (already corrected in the galley proof) listed below –

 

The common ones are –

·       Using “statistically” instead of “statistically significant” multiple times, e.g., in the last sentence of the Abstract, in the 3^rd, 4th and 6^th sentences of the In Vitro Alizarin Red S and Von Kossa Stain in the Results, and so on.

·       Omission of “the” in some places, e.g., before “synthesis” in the 14^th sentence of the 2^nd paragraph of the Introduction, before “spent” in the 2^nd sentence of the 2^nd paragraph of Experimental design in the Materials and Methods, and so on.

·       Omission of “,”, e.g., after “moreover” in the last sentence of the Abstract, after “however” in the 14^th sentence of the 1^st paragraph of the Introduction, and so on.

·       Words like “adipose derived” and “target specific” must be hyphenated.

The other errors are –

 

In the Abstract –

·       In the last sentence, “higher level, statistically different, of osteocalcin” must be replaced with “higher levels (statistically significant) of osteocalcin production”.

In the Introduction –

  In the 1^st paragraph –

·       In the 3^rd sentence, “to” must be replaced with “into”.

·       In the 10^th sentence, “in patient” must be written as a single word.

·       In the 12^th sentence, “to” after “injected” must be replaced with “into”, and “.” Must be replaced with “–”.

·       In the 15^th sentence, “are” after “there” must be replaced with “is”, and “,” after “BMSCs” must be replaced with “;”.

·       In the 17^th sentence, “the” must be removed.

  In the 2^nd paragraph –

·       In the 2^nd sentence, “ASC’s” must be replaced with “ASCs”.

  In the 3^rd paragraph –

·       In the 1^st sentence, “ASC” must be replaced with “ASCs”.

In the Materials and Methods –

  Under ADSC Isolation and Culture in Vitro –

  In the 1^st paragraph –

·       In the 6^th sentence, “the” after “so” must be replaced with “that”.

·       In the 9^th sentence, “a” before “uniform” must be removed.

·       In the 12^th sentence, “were” after “DPBS” must be replaced with “was”, and “re-suspended” must be written without hyphen.

·       In the 14^th sentence, “an” before “hemocytometer” must be replaced with “a”.

  In the 2^nd paragraph –

·       In the last sentence, “,” after “medium” must be removed.

  Under Alizarin Red S Staining –

·       In the last sentence, “statistical multiple” must be replaced with “multiple statistical”, and “comparison” must be replaced with “comparisons”.

  Under Porcine Osteocalcin ELISA –

·       In the penultimate sentence, “Student-t Test” must be replaced with “Student’s t-test”.

In the Results –

  Under In Vitro Alizarin Red S and Von Kossa Stain –

·       In the 3^rd sentence, “but…control” must be replaced with “but there was no statistically significant difference in the number of nodules compared to the standard osteogenic control”.

·       In the 4^th sentence, “significant” must be inserted after “statistically”.

·       In the 6^th sentence, “the” before “vitamin K” must be removed, “significant” must be inserted after “statistically”, and “;” must be inserted after “differences”.

In the Discussion –

  In the 1^st paragraph –

·       In the 1^st sentence, “media’s” must be replaced with “medium’s”.

·       In the 4^th sentence, “ASC” must be replaced with “ASCs”.

·       In the 6^th sentence, “the” before “vitamin” must be removed.

  In the 2^nd paragraph –

·       In the 1^st sentence, “vs” must be followed by “.”.

·       In the 4^th sentence, “was statistically” must be replaced with “were significantly”.

·       In the 7^th sentence, “,” before “however” must be replaced with “;”.

·       In the 9^th sentence, “base osteogenic” must be replaced with “osteogenic base”.

·       In the last sentence, “significant” must be inserted after “statistically”.

Author Info

Bane T.A. Bertels J.C. Matthew B. Wheeler Polkoff K.M. Rubessa M.

Corresponding Author

Matthew B. Wheeler
Department of Animal Sciences, University of Illinois, Urbana-Champaign, Urbana, Illinois, USA

Article Info

Article Type

Research Article

Publication history

Received: Mon 20, Apr 2020
Accepted: Tue 05, May 2020
Published: Mon 11, May 2020

Copyright

© 2023 Matthew B. Wheeler. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Hosting by Science Repository.
DOI: 10.31487/j.JBEM.2020.01.01