A Simple and Convenient Method to Isolate and Culture Rat Aorta Endothelial Cells

Review Data

Q: Is the topic relevant to the journal area of interest? Is it contemporary and interesting for

researchers?

A: Good

Abstract & Keywords

Q: Are all required components included in the abstract? Are the keywords appropriately chosen?

A: Good

Goal

Q: Is the goal explicitly stated in the Introduction? Is its formulation clear and unambiguous?

A: Good

Structure

Q: Is the paper's structure coherent? Is it in coherence with the goal of the paper?

A: Good

Tools and Methods

Q: Are methods the author uses adequate and well used?

A: Good

Discussion & Conclusion

Q: Is it related to the results presented before? Do you consider them as coherent?

A: Good

Comments: The Discussion describes the methods and outcome of the culture of rat aorta endothelial cells providing relevant literature. Further it also highlights on the critical points in executing the method. Lastly, it states that this simple and convenient method to isolate ECs from rat aorta is highly beneficial for the research of endothelium-related diseases.

Literature

Q: Does the author utilize relevant literature?

A: Good

Author's knowledge

Q: What is the level of the author’s knowledge? Does the author utilize all recent contributions relevant to the topic?

A: Good

Length

Q: Is the length of the paper adequate to the significance of the topic? Do you suggest shortening the paper without losing its value?

A: Good

Figures & Tables

Q: Does the author use them suitably? Are legend and notations clear?

A: Good

Writing style

Q: Is it clear and understandable?

A: Good

Further comments on the paper

Comments: This manuscript establishes a simple and convenient method to isolate and culture rat aorta endothelial cells (ECs). This discusses the methods, surgical procedures, isolation and culture of ECs and SMCs followed by immunofluorescence staining.

Q: Would you recommend this manuscript for further publication?

A: Yes - Suitable to be published

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Science Repository Team